Research interests
I am fascinated by how metabolic dependencies emerge, evolve, and shape microbial community structure. With a background in biopolymers and anaerobic mixed-culture fermentations, I initially investigated how strong selective pressures can be used to steer microbial communities toward desired product formation. This experience led me to focus more fundamentally on the ecology and evolution of metabolite cross-feeding, and how these dependencies shape microbial community assembly and spatial organization,
potentially contributing to the emergence of multicellularity. I address these questions through controlled bioreactor experiments, experimental evolution in synthetic co-cultures and mathematical modeling.
potentially contributing to the emergence of multicellularity. I address these questions through controlled bioreactor experiments, experimental evolution in synthetic co-cultures and mathematical modeling.
Education and Scientific Career
Since 2025 Marie Curie & FWO postdoctoral fellow, Center for Microbial Ecology and Technology, Ghent University
2023-2024 Postdoctoral researcher, Center for Microbial Ecology and Technology, Ghent University
2018-2022 PhD in Environmental Biotechnology, Delft University of Technology, The Netherlands
2016-2018 MSc in Chemical and Bioprocess Engineering, Universidade de Santiago de Compostela
2023-2024 Postdoctoral researcher, Center for Microbial Ecology and Technology, Ghent University
2018-2022 PhD in Environmental Biotechnology, Delft University of Technology, The Netherlands
2016-2018 MSc in Chemical and Bioprocess Engineering, Universidade de Santiago de Compostela
Publications
[1] Vulart, L., Estévez, Á., Peiro, E., Gòdia, F. and Ganigué, R., 2025. Combined effect of feeding regime and pH on carboxylic acids production
in thermophilic mixed-culture fermentation. Journal of Environmental Chemical Engineering, 13 (5), 117818.
[2] Pei, R., de Vries, E., Estévez, Á., Sousa, J., Dijkman, H., Tamis, J., Werker, A., 2025. Demonstrating performance in scaled-up production
and quality control of polyhydroxyalkanoates using municipal waste activated sludge. Water Research, 275, 123160.
[3] Werker, A., Pei, R., Kim K., Moretto G., Estévez, Á., Vermeer C., Arcos- Hernandez M., Dijkstra H., and de Vries E., 2023. Thermal pre-processing before extraction of polyhydroxyalkanoates for molecular weight quality control. Polymer Degradation and Stability, 209, 110277.
[4] Estévez, Á., Arias-Buendía, M., Pei, R., van Veelen, H.P.J., van Loosdrecht, M.C.M., Kleerebezem, R. and Werker, A., 2022, Calcium enhances polyhydroxyalkanoate production and promotes selective growth of the polyhydroxyalkanoate-storing biomass in municipal activated sludge. Water Research, 226, 119259.
[5] Estévez, Á., Altamira-Algarra, B., Arnau-Segarra, C., van Loosdrecht, M.C.M., Kleerebezem, R. and Werker, A., 2022, Process conditions affect properties and outcomes of polyhydroxyalkanoate accumulation in municipal activated sludge. Bioresource Technology, 364, 128035.
[6] Pei, R. (1) , Estévez, Á. (1) , van Loosdrecht, M.C.M., Kleerebezem, R., Werker, A., 2022 Exploring the limits of polyhydroxyalkanoate production with municipal activated sludge. Environmental Science & Technology, 56 (16), 11729-11738.
[7] Estévez, Á., van Loosdrecht, M.C.M., Kleerebezem, R. and Werker, A., 2021. Simultaneous nitrification and denitrification in microbial community based polyhydroxyalkanoate production. Bioresource Technology, 337, 125420
[8] Estévez, Á. (1) , Pei, R. (1) , van Loosdrecht, M.C.M., Kleerebezem, R. and Werker, A., 2021. Scaling-up microbial community based
polyhydroxyalkanoate production: status and challenges. Bioresource Technology, 327, 124790.
[9] Mulders, M., Estévez, Á., Stouten, G.R., Tamis, J., Pronk, M. and Kleerebezem, R., 2020. Volatile fatty acid product spectrum as a function
of the solids retention time in an anaerobic granular sludge process. Journal of Environmental Engineering (US), 146(8), 04020091.
in thermophilic mixed-culture fermentation. Journal of Environmental Chemical Engineering, 13 (5), 117818.
[2] Pei, R., de Vries, E., Estévez, Á., Sousa, J., Dijkman, H., Tamis, J., Werker, A., 2025. Demonstrating performance in scaled-up production
and quality control of polyhydroxyalkanoates using municipal waste activated sludge. Water Research, 275, 123160.
[3] Werker, A., Pei, R., Kim K., Moretto G., Estévez, Á., Vermeer C., Arcos- Hernandez M., Dijkstra H., and de Vries E., 2023. Thermal pre-processing before extraction of polyhydroxyalkanoates for molecular weight quality control. Polymer Degradation and Stability, 209, 110277.
[4] Estévez, Á., Arias-Buendía, M., Pei, R., van Veelen, H.P.J., van Loosdrecht, M.C.M., Kleerebezem, R. and Werker, A., 2022, Calcium enhances polyhydroxyalkanoate production and promotes selective growth of the polyhydroxyalkanoate-storing biomass in municipal activated sludge. Water Research, 226, 119259.
[5] Estévez, Á., Altamira-Algarra, B., Arnau-Segarra, C., van Loosdrecht, M.C.M., Kleerebezem, R. and Werker, A., 2022, Process conditions affect properties and outcomes of polyhydroxyalkanoate accumulation in municipal activated sludge. Bioresource Technology, 364, 128035.
[6] Pei, R. (1) , Estévez, Á. (1) , van Loosdrecht, M.C.M., Kleerebezem, R., Werker, A., 2022 Exploring the limits of polyhydroxyalkanoate production with municipal activated sludge. Environmental Science & Technology, 56 (16), 11729-11738.
[7] Estévez, Á., van Loosdrecht, M.C.M., Kleerebezem, R. and Werker, A., 2021. Simultaneous nitrification and denitrification in microbial community based polyhydroxyalkanoate production. Bioresource Technology, 337, 125420
[8] Estévez, Á. (1) , Pei, R. (1) , van Loosdrecht, M.C.M., Kleerebezem, R. and Werker, A., 2021. Scaling-up microbial community based
polyhydroxyalkanoate production: status and challenges. Bioresource Technology, 327, 124790.
[9] Mulders, M., Estévez, Á., Stouten, G.R., Tamis, J., Pronk, M. and Kleerebezem, R., 2020. Volatile fatty acid product spectrum as a function
of the solids retention time in an anaerobic granular sludge process. Journal of Environmental Engineering (US), 146(8), 04020091.